ZFIN Image: García-Castillo et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Telomerase RNA increases the efficiency of in vitro transcription. (A) In vitro transcription efficiency of csf3b promoter-driven luciferase reporter in the presence of 25 nM GFP, terc RNA, or no RNA as control, using nuclear extracts from HL60 cells as the source of the transcriptional machinery. Data are the average of at least three independent experiments. luc, luciferase. (B) Same as in A, using recombinant proteins as source of the transcriptional machinery. (C) In vitro transcription efficiency of csf3b promoter-driven luciferase reporter in the presence of 10 nM GFP, terc, CR4-CR5M mutant terc RNA, or no RNA as control, using nuclear extracts from HL60 cells as the source of the transcriptional machinery. Data are the average of at least three independent experiments. (D) Same as in C, using recombinant proteins as source of the transcriptional machinery. Data values shown for all panels are fold change over the no RNA control sample. All data are mean + SEM. **P < 0.01 and ***P < 0.001 for one-way ANOVA plus Bonferroni posttest in A, B, C, and D. ns, not significant.

Acknowledgments

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