Figure 2

Figure 2

Apoptotic cell death in the reducing LMFF area. (a–c’’) Acridine orange staining in the reducing LMFF area (a’,b’,c’) and proximal part of the developing caudal fin region (a”,b”,c”) at 6.0–6.5 mm SL (a–a”), 6.5–7.0 mm SL (b–b”), and 7.0–7.5 mm SL (c–c”). The lower panels (a’,a”,b’,b”,c’,c”) are magnified views of the dashed rectangles in the upper panels (a,b,c), respectively. (d–f”) Expression pattern of active caspase 3 in the reducing LMFF area (d’,e’,f’) and proximal part of the developing caudal fin region (d”,e”,f”) area at 6.0–6.5 mm SL (d–d”), 6.5–7.0 mm SL (e–e”), and 7.0–7.5 mm SL (f–f”). The lower panels (d’,d”,e’,e”,f’,f”) are magnified views of the dashed rectangles in the upper panels (d,e,f), respectively. Arrowheads in (a”,b”,c”,d”,e”,f”) indicate examples of apoptotic cell death signals. Scale bars indicate 200 μm.