FIGURE 6

FIGURE 6

The effect of miR-29b-3p on the expression of DNMTs. (A and B) The predicted binding site of miR-29b-3p in the 3′ untranslated regions of DNMT3A and DNMT3B. (C and D) The relative luciferase activity of HL1 cells cotransfected with miR-29b-3p mimic or NC mimic and plasmid containing DNMT3A or 3B wild-type or mutated 3′UTRs. (miR-29b-3p mimic + psiCHECK™-2-DNMT3A/3B-3′UTR vs. NC mimic + psiCHECK™-2-DNMT3A/3B-3′UTR, ***p < 0.001; miR-29b-3p mimic + psiCHECK™-2-DNMT3A/3B-3′UTR-MUT vs. NC mimic + psiCHECK™-2-DNMT3A/3B-3′UTR-MUT, p = ns). (E) mRNA expression of DNMTs in HL1 cells transfected with miR-29b-3p mimic or its inhibitor (miR-29b-3p mimic vs. NC, *p < 0.05, miR-29b-3p inhibitor vs. NC inhibitor, ***p < 0.001). (F) Protein expression of DNMTs in HL1 cells transfected with miR-29b-3p mimic or its inhibitor. (G) Relative quantification of DNMT proteins (^ns not significant, *p < 0.05, **p < 0.01 and ***p < 0.001).