IMAGE

Figure 6

ID
ZDB-IMAGE-220416-60
Genes
Source
Figures for Huang et al., 2022
Image
Figure Caption

Figure 6 Granulocytic differentiation at late developmental stages and in adulthood also shows defects in nelfb−/− zebrafish. (A) WISH for runx1 or rag1 in WT and nelfb−/− embryos at 26 hpf and 4 dpf. (B) WISH for cmyb in WT and nelfb−/− embryos at 2 dpf. (C) WISH for mpx, mfap4, and hbbe1 in WT and nelfb−/− embryos at 3 dpf. Blue arrowheads, white arrowheads, and red arrowheads, respectively, indicate AGM region, thymus, and CHT. (D) Quantification of WISH results in (A–C) (n = 30–40 embryos per group). (E) WISH for mpx at 3 dpf in WT and nelfb−/− embryos treated with flavopiridol. (F) Quantification of WISH results in (E) (n = 20–30 embryos per group). (G) WISH for mpx at 3 dpf in WT and nelfb−/− embryos injected with 1.2 ng cdk9 MO. (H) Quantification of WISH results in (H) (n = 20–30 embryos per group). (I) Q-RT-PCR analysis of 5′ and 3′ transcripts of mpx gene in WT and nelfb−/− embryos at 5 dpf. Gene expression is normalized to the 5′ transcript of β-actin and shown as fold-change relative to WT. (J) Q-RT-PCR analysis of hematopoiesis-related genes in WT and nelfb−/− mutant fish at 26 dpf. Gene expression is normalized to β-actin and presented as fold-change relative to WT. (K) May–Grünwald–Giemsa staining of WKM from WT and nelfb−/− fish at 8 mpf. Blue arrowheads, precursors; green arrowheads, intermediate myelomonocytes; yellow arrowheads, mature neutrophils; black arrowheads, lymphocytes and HSCs; red arrowheads, erythrocytes. (L) Quantification of WKM staining results. All results are presented as the mean ± SD from a representative of three independent experiments (t test, * for p < 0.05, ** for p < 0.01, *** for p < 0.001). “+++” and “+” in (D,F,H) respectively represent strong staining and weak staining. Note: dpf, days post-fertilization; mpf, months post-fertilization; AGM region, aorta-gonad-mesonephros region; CHT, caudal hematopoietic tissue; WKM, whole kidney marrow; HSCs, hematopoietic stem cells.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.