Figure 5.

Figure 5.

miR-22a directly targets sema4c. (a) Venn diagram of predicted target genes of miR-22a and transcriptomic upregulated genes. (b) Sema4c 3′-UTR target sites of miR-22a. (c) Overexpression of miR-22 (miR-22-pre) reduced sema4c-3′-UTR luciferase activity in HeLa cells (n = 9). Data are expressed as mean ± s.e. t-test; **p < 0.01. (d) EGFP sensors were co-injected with mCherry control as indicated. miR-22a-precursor injection reduced the EGFP levels in EGFP-sema4c-3′-UTR sensor (second column), whereas mCherry levels were unchanged. In the mutated sensor, no reduction in GFP was noted (experiments were repeated three times; for each group, around 10 embryos were analysed).