ZFIN Image: Sheng et al., 2022, Figure 6<tag0:x xml:space='preserve'>. </tag0:x>

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Figure 6.

Reducing sema4c partially restored the defects of ISVs and PMNs in miR-22a-deficient embryos. (a) Confocal imaging analysis of blood vessels in control, miR-22a-MO, and miR-22a-MO + sema4c knockdown embryos at 30 hpf. (b) Statistics of horizontal sprouts ratio in control (n = 7), miR-22a-MO (n = 7) and miR-22a-MO + sema4c knockdown embryos (n = 7) at 30 hpf. One-way ANOVA; ***p < 0.001; ****p < 0.0001. (c) Confocal imaging analysis of control, miR-22a-MO and miR-22a-MO + sema4c knockdown Tg(mnx1:EGFP) embryos at 72 hpf. (d) The statistical analysis of the ratio of aberrant axonal projection of CaPs in the control (n = 8), miR-22a morphants (n = 8) and miR-22a-MO + sema4c knockdown embryos (n = 8) at 72 hpf; one-way ANOVA, ****p < 0.0001.

Acknowledgments

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