Figure 5—figure supplement 1.

Figure 5—figure supplement 1.

(A) Schematic diagram showing the core components of the mammalian STRIPAK complex and some of its accessory molecules. Core STRIPAK components include serine/threonine-protein phosphatase catalytic subunit known as PP2Ac and the scaffolding subunit PP2Aa, together with striatins (STRN, STRN3, and STRN4), Mob3, STRIP1, or STRIP2, and a germinal center kinase, GCKIII kinase, bound via cerebral cavernous malformation 3 (Ccm3). Some molecules bind in a mutually exclusive pattern to the core components. Arrows show that sarcolemmal membrane-associated protein (SLMAP) and the suppressor of IKKe (SIKE) are not detected in STRIPAK complexes containing cortactin-binding proteins (CTTNBP2/NL) and vice versa. (A has been adapted from Figure 3 in Hwang and Pallas, 2014). (B) STRING protein–protein interaction (PPI) network analysis of six zebrafish Strip1-interacting partners significantly enriched from IP-MS analysis highlighted in Figure 5C. Network edges represent known and/or predicted functional interactions in the STRING database. Edge thickness reflects the combined STRING evidence score for each binary relationship. Thicker edges represent increased interaction evidence. PPI enrichment p value <1.0e−16. (C) Uniform Manifold Approximation and Projection (UMAP) plots showing different clusters of retinal cells at 48 hpf analyzed with Seuret R pipeline using data published by Xu B. et al. (2020). Twelve clusters are identified and categorized to different retinal cell types or subtypes. RPCs, retinal progenitor cells; RGCs, retinal ganglion cells; ACs, amacrine cells; BPs, bipolar cells; HCs, horizontal cells; PRs, photoreceptors; MGs, Müller glia. UMAP plots showing retinal mRNA expression patterns of strip1 (D) and its interacting STRIPAK components, identified from Co-IP/MS; cttnbp2 (E), ttnbp2nlb (F), strn (G), strn3 (H), and strn4 (I). Only strip1 and strn3 show high retinal expression levels at 48 hpf compared to other partners.