Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

(A) 3D confocal live images showing neurite projection of ACs to the IPL in transplantation assays outlined in Figure 4B, C. Panels represent three combinations of transplantation experiments: from wild-type donor to wild-type host (left columns), from strip1^rw147 mutant donor to wild-type host (middle columns), and from wild-type donor to strip1^rw147 mutant host (right columns). Donor cells are labeled with dextran-Alexa-488 in green and ACs are labeled with ptf1a:mCherry-CAAX in magenta. Mutant donor ACs show a normal dendritic pattern and normal projection to the IPL in wild-type host retina, although on rare occasion we observed that mutant ACs display two dendritic trees (arrow in the bottom middle panel), but still contribute to a normal IPL. On the other hand, wild-type donor ACs transplanted into mutant host retinas show abnormal projection patterns and infiltrate the ganglion cell layer (GCL; arrowheads in the right panels). Scale bar, 20 μm. (B) Confocal image of 3-dpf live wild-type and strip1^rw147 mutant retinas combined with the transgenic line, xfz43, to label subsets of BPs. Scale bar, 10 μm. (C) Schematic drawings of cell transplantation design to evaluate cell autonomy of Strip1’s role in BP development. strip1^rw147 mutant donor cells carrying the transgene xfz43 (green) are labeled with dextran-cascade blue or dextran-Alexa-flour 647 (blue) and transplanted into wild-type host embryos at blastula stage, leading to chimeric wild-type retina with strip1^rw147 mutant retinal columns. Retinal lamination of the host retina is visualized with Bodipy TR (magenta). (D) 3D confocal live images of four wild-type host retinas with strip1^rw147 mutant donor retinal columns outlined in (C). strip1^rw147 mutant donor BPs show normal neurite projection patterns to the IPL in wild-type host retina. Occasionally, bipolar axon terminals are abnormally extended laterally (arrow in the bottom panel); however, such defects are confined to a seemingly normal IPL. Scale bar, 10 μm. (E) Confocal image of 3-dpf live wild-type and strip1^rw147 mutant retinas combined with the transgenic line, xfz3, to label subsets of BPs. Scale bar, 10 μm. (F) Schematic drawings of cell transplantation design to evaluate the cell autonomy of Strip1’s role in BP development. Wild-type donor cells carrying the transgene xfz3 (green) are labeled with dextran rhodamine (magenta) and transplanted into strip1^rw14 mutant host embryos at blastula stage, leading to chimeric strip1^rw147 mutant retinas with wild-type retinal columns. (G) 3D confocal live images of two different strip1^rw147 mutant host retinas with wild-type donor retinal columns outlined in (F). Wild-type donor BPs show misguided neurite projections. Scale bar, 10 μm.