ZFIN Image: Lu et al., 2022, Fig 7

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Figure Caption

Fig 7

Fig 7. Macrophage/microglia function is required for the maintenance of mTOR activity in an inflammation-independent manner.

(A,J) Schematic of the experimental paradigm showing the timeline for chemical treatments and ablation on transgenic larvae (mpeg1:mCherry;rpe65a:nfsB-eGFP). (B-G) MTZ⁺ DMSO- and PLX3397-treated larvae at 2dpi. Single channel immunofluorescent images of mCherry (D,E) and p-S6 (F,G) are shown. (B’,D’,F’) are high-magnification images showing the colocalization of p-S6 and mCherry. Nuclei (white), eGFP (green), p-S6 (magenta), mCherry (yellow). (H) Quantification of mCherry signal in the RPE layer showed a significant depletion of mCherry⁺ macrophages/microglia in the RPE after PLX3397 treatment in MTZ⁺ larvae, compared to DMSO-treated controls. (I) Quantification of p-S6 levels in the RPE layer showed a significant decrease in PLX3397-treated larvae, when compared to DMSO-treated controls. (K-N) Fluorescent images of p-S6 immunostaining on cryosections from MTZ^- and MTZ⁺ DMSO- and Dex-treated larvae at 7dpf/2dpi. Nuclei (white), eGFP (green), p-S6 (magenta). (O) Quantification of p-S6 level in the RPE layer showed a significant increase in MTZ⁺ DMSO-treated larvae when compared to MTZ^- DMSO-treated control, but no significant differences between Dex-treated and DMSO-treated larvae from MTZ^- or MTZ⁺ groups. p-values: ** ≤ 0.01, *** ≤ 0.001. Statistical information can be found in S9 Table. Dorsal is up and distal is left. Scale bar = 50μm.

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