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Figure 4—figure supplement 1. Maternal zygotic <italic toggle='yes'>ryk<sup>nce4g</sup></italic> mutants present Convergent Extension defects.

(A,B) ryk morphants present Convergent Extension (CE) defects that can be rescued by WT ryk (A) but not ryknce4g mutant (B) RNA. (C) CE is similar in Zygotic (Z) ryknce4g mutants and their WT siblings. (D) In situ hybridization reveals that ryk transcript levels are reduced in Z ryknce4g mutants (n = 24) compared to WT siblings (n = 24). 12 somite stage embryos, anterior to the left, dorsal up. To warrant identical acquisition conditions, two embryos were photographed on a single picture. (E–F) foxa3 in situ hybridization shows that Maternal Zygotic (MZ) ryk mutants present a reduced axial elongation (E) and an increased width of the notochord (F, for quantification see Figure 4I). (G) qPCR analysis of bud stage embryos reveals that MZ ryknce4g mutants present reduced ryk transcript levels. (H) In contrast to Z ryknce4g mutants, Maternal Zygotic (MZ) ryknce4g mutants present CE defects. To exclude any defects due to genetic background variation, the parental fish used to obtain the embryos for this experiment were ryk[+/+] and ryk[nce4g/nce4g] siblings obtained from the same incross. ryk WT RNA injection allows to rescue MZ ryknce4g mutant CE defects. (I) ryk morpholino injection has no effect in MZ ryknce4g mutants. Lateral (A,B,C,E,H,I) or dorsal (F) views of bud stage embryos, anterior up. Scalebars 200 µm. Boxes in (E,I) boxes represent mean values ± SD. Error bars in G represent SE from three biological replicates. Quantitative analysis of the data sets displayed in (A,B,C,H) is provided in Figure 4D, E, G and H respectively. See Figure 4—source data 1 for complete statistical information.

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