Image
Figure Caption

Figure 1—figure supplement 1. Mib1 knock-down impairs axial elongation.

(A,B) Axis elongation was quantified at bud stage by measuring the axis extension angle α using the marker genes shhb (A, expressed in the notochord) and foxa3 (B, expressed in notochord and prechordal plate). Axis extension is reduced following injection of mib1 morpholino (MO mib1) or RNAs encoding Mib1ΔRF123 or Mib1ΔRF3. Lateral views of bud stage embryos, anterior up, dorsal to the right. (C) Dorsal views of bud stage embryos stained for foxa3 also reveal a significant widening of the Notochord. Boxes represent mean values ± SD. See Figure 1—source data 1 for complete statistical information.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife