ZFIN Image: Okada et al., 2022, Figure 3.

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Figure Caption

Figure 3.

Dose-dependent cleavage efficiencies of selected crRNAs from low, medium, and high activity groups. Two crRNAs each from high (A), medium (B) and low (C) activity groups were injected as RNP at four different amounts of 0.75, 1.5, 3 and 4.5 fmol. Indel frequencies (in %) assessed with ICE for RNP complexes assembled with WT Cas9 and HiFi Cas9 are plotted on the left and right sides, respectively. Means of replicates are shown with standard error bars. The experimental dataset is shown in Supplementary Table S5.

Acknowledgments

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