ZFIN Image: Okada et al., 2022, Figure 1.

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Figure Caption

Figure 1.

Establishing adequate quantities of CRISPR-Cas9 RNP complexes for crRNA evaluation. (A, B) Indel frequencies (in %) generated by different amounts of RNP complex with WT Cas9. 1 nl and 2 nl of 1.5 μM RNP complex (1.5 and 3 fmol, respectively) assembled using WT Cas9 with the indicated crRNAs were microinjected into 1-cell stage embryos, and indel frequencies were assessed with TIDE (A) and ICE (B). (C, D) Indel frequencies (in %) generated by different amounts of RNP complex with HiFi Cas9. TIDE (C) and ICE (D) were used for the assessment.

Acknowledgments

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