IMAGE

Fig 6

ID

ZDB-IMAGE-220318-7

Genes

EGFP
mRFP

Publication

Nandamuri et al., 2022 - Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma

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Figures for Nandamuri et al., 2022

Image

Figure Caption

Fig 6 <italic toggle='yes'>Tg(sox10</italic>:<italic toggle='yes'>memRFP)-</italic>positive neural crest cells do not properly localize in the optic fissure in <italic toggle='yes'>dzip1</italic> mutants.

Embryos visualized for neural crest (green; Tg(sox10:memRFP)) and cell membranes (magenta; Tg(bactin2:EGFP-CAAX)) in wild type (A-C) and dzip1^ts294e mutants (D-F) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). (A’-F’) Zoomed views of images in A-F to facilitate visualization of neural crest cells within the optic fissure. All images are lateral views of 3-dimensional renderings. Some Tg(sox10:memRFP)-positive neural crest cells are detectable in the optic fissure by 24 hpf in both wild type and dzip1^-/- embryos (white arrowheads in A, D). (G-I) Quantification of total number of Tg(sox10:memRFP)-positive cells through the length of the optic fissure within the eye at 24, 36, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One