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Figure 1 Experimental design and behavioural assessments. (A) Zebrafish eggs were collected immediately after fertilization and microinjected with either 0, 1 or 10 ng venlafaxine. Eggs were raised to either 60 dpf, and assessed for behaviour, or until 10–12 months post-fertilization and assessed for behaviour, metabolic rate, and swim performance. A top-down camera view was utilized for Novel tank (B) and Novel object (C) assessments. An air stone (blue) was the novel object (C) placed in the aquarium. (D) Lateral view behavioural assessment was carried out with the camera placed on the same surface of the tank of interest (D). The images were created with BioRender.com.