IMAGE

Fig 7

ID
ZDB-IMAGE-220227-31
Genes
Antibodies
Source
Figures for Berger et al., 2022
Image
Figure Caption

Fig 7 The myofibril defects of <italic toggle='yes'>tmod4</italic>-deficient mutants are distinct from the defects of <italic toggle='yes'>lmod3-</italic> and <italic toggle='yes'>capza1b</italic>-deficient mutants.

(A) After rescaling to WT siblings (100 ± 1%), the significant reduction of birefringence of single lmod3sa13018 (78 ± 1%) and capza1bex5 (79 ± 1%) homozygotes was further reduced in lmod3sa13018/sa13018;capza1bex5/ex5 compound homozygotes (61 ± 2%). (B) At 4 dpf, lmod3sa13018;capza1bex5 compound homozygotes featured thin filament deposits at the peripheral ends of myofibres (arrowhead), which were also highlighted in single capza1bex5 homozygotes by Lifeact-GFP (green) and mCherry-CaaX (red). The strong myofibril striation seen in WT siblings appeared severely reduced in capza1bex5 homozygotes and was rarely detected in lmod3sa13018;capza1bex5 compound homozygotes (arrow). (C) After rescaling to WT siblings (100.0 ± 0.9%), the significant reduction of birefringence of single lmod3sa13018 (82 ± 1%) and tmod4trg homozygotes (47 ± 1%) was further reduced in lmod3sa13018;tmod4trg compound homozygotes (30 ± 1%). (D) At 3 dpf, the typical myofibril striation was highlighted by antibodies against actinin (green) and actin-labelling with phalloidin (red). In lmod3sa13018;tmod4trg compound homozygotes striation was rarely seen (arrowhead). Instead, actin- and actinin-positive aggregates were detected throughout myofibres (arrow) as well as actin-positive deposits close to vertical myosepta (double arrow). Data are presented as mean ± SEM; *** P < 0.001 calculated by one-way ANOVA with post hoc Tukey’s test; n = 5 clutches. Scale bar sizes are 20 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.