ZFIN Image: Zhang et al., 2022, Fig. 5

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Figure Caption

Fig. 5 Variation of the fgf8 mRNA concentration with somite stage.

Negative ln(<[fgf8m]>) (=ln(2) δCt values from the RTqPCR data) at different somitic stages (blue ○: whole embryo; red ◊ : PSM only). Red line: linear best fit (y = ax + b) with a = 0.094 ± 0.013 (Χ²= 22; DF = 36), corresponding to a an exponential decrease of <[fgf8m]> with a time constant τ = 1/a ≅ 11 somitic periods, Eq. (1). Green ◊: -ΔPSM/λ with λ = 260 μm and the PSM shrinkage, ΔPSM, taken from Fig. 4e. From the value of λ and τ we deduce a mean velocity: V_PSM = λ/τ ≅ 24 μm/somite.

Acknowledgments

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