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Fig. 5.
Effects of pharmacological PPAR, LXR, and RXR modulation on apoeb and apoc1 expression in the developing zebrafish CNS. (A) Experimental design for treatment by immersion with selected compounds. After 24 h of treatment, whole heads were removed and pooled for each group for RNA extraction and cDNA synthesis. (B) Effects of PPAR-RXR agonist LG100754 on the indicated genes measured by qPCR. (C) Effects of LXR agonist GW3965 on the indicated genes measured by qPCR. (D) Effects of RXR antagonist HX531 on the indicated genes measured by qPCR. In all experiments, the vehicle treated (DMSO) group served as control. Each dot represents the value from one sample of pooled heads (3–9 heads pooled per sample). Statistically significant differences are indicated (***P<10−4, **P<0.01, *P∼0.02). Image in A was generated in BioRender.