IMAGE

FIGURE 5

ID
ZDB-IMAGE-220203-40
Genes
Source
Figures for Wu et al., 2022
Image
Figure Caption

FIGURE 5

Gfi1aa targets etv2 and sox7 in an Lsd1-dependent manner (A,B) Gfi1 repression activity on etv2 and sox7 transcription depends on Lsd1. Expression of etv2 (A) and sox7 (B) in WT, lsd1 MO, gfi1aa overexpression (gfi1aa-OE) embryos, and gfi1aa-OE embryos co-injected with 1 pmol lsd1 MO. gfi1aa-OE embryos were the progenies of hsp-gfi1aa-eGFP transgenic fish. The red boxes indicate the magnification of etv2 signals (A) and sox7 signals (B) in the ICM region. n ≥ 10 embryos for each group. The numbers in the bottom right corner indicate the percentage of embryos exhibiting the representative expression of indicated genes. Scale bar: 200 μm (C,D) Expression (C) and analysis (D) of erythroid marker alas2 in sibling, gfi1aa −/− mutant, gfi1aa-OE rescued gfi1aa −/− mutant and gfi1aa −/− mutant with gfi1aa-OE and lsd1-MO at 22 hpf (E,F) Expression (E) and analysis (F) of endothelial marker flk1 in sibling, gfi1aa −/− mutant, gfi1aa-OE rescued gfi1aa −/− mutant and gfi1aa −/− mutant with gfi1aa-OE and lsd1-MO at 22 hpf. The red boxes indicate the magnification of ICM region, and the red arrows indicate WISH signals (****p < 0.0001, **p < 0.01, *p < 0.05, ns, no significant, Fisher exact tests, n ≥ 10 embryos for each group). Scale bar: 200 μm (G,H) H3K4me1 levels at etv2 intron-2 locus and sox7 promoter were inhibited by Gfi1aa. ChIP-qPCR showed H3K4me1 level at etv2 gene loci (G) and sox7 promoter (H) in AB and gfi1aa −/− mutant embryos (The error bars represent three technical replicates and two independent experiments were performed, mean ± SEM; **p < 0.01; t-test).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol