ZFIN Image: Prendergast et al., 2022, Figure 3

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Figure Caption

Figure 3

Knocking out Ehlers-Danlos and Marfan Syndrome-related genes generates vascular phenotypes. (A) Brightfield macroscopic image of representative series of uninjected larvae at 3 dpf. Scale bar: 1 mm. (B) Similar image of fbn1 knockout fish. Filled arrowhead indicates cardiomegaly. (C) Similar image of col1a2 knockout fish. (D) Similar image of col5a1 knockout fish. (E) Similar image of col5a2a/b knockout fish. (F) Closeup brightfield image of a 6 dpf col5a1 knockout fish with visible aortic hemorrhage (filled arrowhead, scale bar: 500 µm). (G) Confocal micrograph of trunk vasculature of a 4 dpf uninjected Tg(kdr:gfp) fish. DA: dorsal aorta, ISV: intersegmental vessel. Scale bar: 100 µm. (H) Similar micrograph of a col5a1 knockout fish. Empty arrowheads indicate b: excessively branching segment and m: missing segments. (I) Quantification of DA diameter. Diameter is not significantly changed across conditions (t-test, p = 0.998). (J) Quantification of ISV diameter. Diameter is not significantly changed across conditions (t-test, p = 0.342).

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Phenotype details

Acknowledgments

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