ZFIN Image: Wang et al., 2021, FIGURE 5

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Figure Caption

FIGURE 5

The effect of overexpression of DM-lepa on cells. (A,B) Cell proliferation was determined by assay of CCK8. (C) Apoptosis was evaluated with Hoechst/PI staining. PI stains dead cells or late-stage apoptotic cells with a red fluorescence, and Hochest stain the nuclei with a bright-blue fluorescence. (D) The percentage of apoptotic cells was determined using ImageJ software. (E) Protein levels of cleaved-caspase 3 within cells was detected by western blot analysis.β-actin as the loading control. (F) ROS was detected by flow cytometry and analyzed using FlowJo software. (G) The relative expression of ROS Data were analyzed and graphed using GraphPad Prism 8. The results are presented as mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001.

Acknowledgments

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