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Figure 1 Macroscopic features of gut explants from zebrafish under (A) resting conditions, (B) after 8 h of treatment with 0.5% dextran sodium sulfate (DSS), (C) after 16 h of treatment with 0.12 mg/Ml STW 5, and (D) after DSS + STW 5 treatment. (E–G) Tissue sections of zebrafish gut (bulb) stained with Alcian blue showing the distribution and density of goblet cells (blue) under resting conditions, DSS- or DSS+STW5-treatment. (H–J) Relative number of goblet cells in bulb, midgut, and hindgut explants. The quantification was performed using five different slides of each part of the intestine from three animals (n = 15 sections total for bulb, midgut, and hindgut). Data are expressed as the mean number of goblet cells ± standard deviation (SD). Statistical significance was calculated using the Wilcoxon test comparing DSS-treated or DSS+STW 5–treated samples with controls. * p ≤ 0.05; ** p ≤ 0.01. DSS+STW 5–treated samples vs. DSS (• p ≤ 0.05; •• p ≤ 0.01).