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Figure 6 Effect of DSF on hatching and deformity rate in wild-type (WT) and cystinotic (KO) zebrafish embryos and larvae. (A) Representative microscopy image of WT (a) and KO (b) zebrafish embryos. Scale bar: 1 mm. (B,C) Hatching and deformity rates in embryos and larvae treated with different doses of DSF (WT: n = 116 and 104 at 10 and 100 μM, respectively; KO: n = 91, 85, 68, 58, and 17, at 0.5, 1, 5, 10, and 50 μM, respectively). Deformity rates were evaluated after 96 hpf. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to untreated embryos or larvae.
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