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Figure 1—figure supplement 2—source data 1. Asymmetry of lratd2a-expressing dHb neurons.(A) Dorsal views of the habenular region of three Tg(lratd2a:QF2); Tg(QUAS:GFP) larvae at 7 dpf. Each confocal image is a single focal plane (1 μm). Brackets indicate regions of lratd2a-expressing neurons in the dHb and arrowheads point to the bilateral vHb. Scale bar, 25 μm. (B) Quantification of lratd2a-expressing dHb neurons. Each line connecting the circles corresponds to a single larva. Mean is 13.1 ± 1.4 cells in the left dHb and 33.5 ± 2.3 cells in the right, n = 15 larvae [p < 0.0001, paired t-test].

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