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Fig. 1.
Injection of ATG poly-GR evokes apoptosis and aberrant motor neuron axon morphology in 1-4-dpf zebrafish embryos. (A) Maximum projection of z-stack images of the SecA5 fluorescent reporter line (green) embryos 48 h after injection with 10 pg ATG poly-GR, 10 pg TAG poly-GR or 400 pg mCherry only. (B) Quantification of z-stack images of the SecA5 fluorescent reporter line embryos after injection with 10 pg ATG poly-GR, 10 pg TAG poly-GR or 400 pg mCherry only at 1-4 dpf. N=minimum of ten fish per group (mean±s.e.m.). P<0.0001 (Kruskal–Wallis test). *P<0.05, ***P<0.0001 (Dunn's post-hoc multiple comparison test), differences of ATG poly-GR-injected fish compared to 10 pg TAG poly-GR and mCherry only at 1-4 dpf. (C) Synaptic vesicle (SV2) in combination with α-bungarotoxin (BTX) staining demonstrates aberrant axonal protrusions in the tail of 2-dpf wild-type AB embryos after injection with 10 pg ATG poly-GR compared to 10 pg TAG poly-GR and 400 pg mCherry only. n=10 per group (mean±s.e.m.). (D) Fluorescence of SV2 staining was measured for five fish/group and three neurites per fish, and was significantly reduced in 10 pg ATG poly-GR-injected embryos. P<0.0001 (one-tailed t-test with Welch's correction). Scale bars: 100 µm.