ZFIN Image: Bhatia et al., 2021, Figure 2

Image

Figure Caption

Figure 2

Characterisation of SHH-SBE2 landing line.

(A) Top: schematic of the design of the landing pad bearing SHH-SBE2 as the tracking cis-regulatory element (CRE). Below: CRE activity observed exclusively in the forebrain in F1 embryos with the SHH-SBE2-eGFP tracking cassette. Images shown for pool of F1 embryos (scale bar = 1000 μm) and individual embryos (scale bar = 100 μm) at different stages of embryonic development. FB, forebrain; hpf, hr post fertilisation. Scale bar = 100 μm. (B) Unique bands (*) observed in round 2 of PCR amplification of DpnI digested genomic DNA from F1 embryos bearing the landing pad cassette. (C) Ensembl Genome Browser snapshot depicting the integration site (red arrow) of the SHH-SBE2 landing pad and sequencing data from clones bearing the PCR product shown by * in (B).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife