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Fig. EV6 PCR proof of a 700 bp deletion in the chd-7 gene in chd-7(gk290) mutant worms. Lifespan analyses of chd-7(gk290) Caenorhabditis elegans mutants treated with ephedrine (green) compared with control DMSO (black). Log-rank test was performed for statistical analyses. (N = 3, n = 50; *P < 0.05). Survival rate of chd7−/− zebrafish mutants treated with ephedrine (blue) compared with untreated mutants (red). N = 3, n = 60. Acetylated tubulin staining in non-treated and ephedrine-treated chd7−/− zebrafish mutants showing rescue of the severely affected outbranching structure of Vth cranial nerves. Graphs showing quantitative analyses of percentage and mean total length of peripheral projections per zebrafish in controls and mutants without and with ephedrine treatment (n = 5; ***P < 0.001; **P < 0.005; one-way ANOVA). Data information: Data are presented as mean ± SEM. Scale bar = 50 μm. n is the number of fish or worms used. N is the number of experimental repeats.