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Fig. EV 3 A, B. Immunostaining with BrdU and HuC/D in brain sections of the zebrafish tectal region in chd7+/+ (A) and chd7−/− (B). Level of the sections is indicated in the sketch of a 5 dpf zebrafish brain (top right image in (A)). The scale bar is 10 μm. Tel: Telencephalon; TeO: tectum opticum, m: medial tectal proliferating zone, DT: dorsal thalamus, PTd: dorsal part of posterior tuberculum, PTv: ventral part of posterior tuberculum, l: lateral tectal proliferation zone. Asterisks (*) marks early migrated region of pretectum and proglomerular. C. The number of BrdU-positive cells in transverse sections of the zebrafish brain in chd7+/+ and chd7−/− (N = 3, chd7+/+: n = 8; chd7−/−: n = 4; **P < 0.05; Student’s t-test). D. Immunostaining with BrdU and HuC/D in brain sections of the zebrafish medial tectal region. Scale bar = 10 μm. m: medial tectal. E. The percentage of BrdU and HuC/D-double positive cells among the BrdU-positive cells in the medial tectal zone (N = 3, n = 4; **P < 0.05; Student’s t-test). F. Immunostaining with BrdU and GFP (to label dlx5a/6a-GFP + GABAergic neurons) in brain sections of the zebrafish medial tectal region. Scale bar = 10 μm. m: medial tectal. G. The percentage of BrdU and dlx5a/6a-GFP-double positive cells among the BrdU-positive cells in the medial tectal zone (N = 3, n = 3; **P < 0.05; Student’s t-test). Data information: Data are presented as mean ± SEM. n is the number of fish used. N is the number of experimental repeats.