ZFIN Image: Ghanta et al., 2021, Figure 1

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Figure Caption

Figure 1

5′-End-modified donors promote homology-directed repair (HDR) in traffic light reporter (TLR) cells.

(A) Schematic showing the TLR assay to quantify HDR efficiencies using unmodified or end-modified double-stranded DNA (dsDNA) donors. Editing efficiencies plotted as percentage of (B) green fluorescent protein (GFP)+ (HDR) and (C) mCherry+ (non-homologous end joining [NHEJ]) HEK293T TLR cells at different amounts of unmodified, 2′-O-methyl (2′OMe)-RNA::triethylene glycol (TEG)-modified dsDNA donors. Editing efficiencies plotted as percentage of (D) GFP+ (HDR) and (E) mCherry+ (NHEJ) HEK293T TLR cells at 1.2 pmol of dsDNA donors indicated. Percentage of GFP+ cells obtained with dsDNA donors modified with various lengths of ethylene glycol (F) and with modifications to only one end or both 5′-ends of the donor. TS, target strand; NTS-,non-target strand (G). Mean ± s.d. for at least three independent replicates are plotted; two replicates for TEG donor in panel G.

Acknowledgments

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