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Fig s1 YPEL3 is evolutionarily conserved and expressed within the developing CNS and in the motor nerve. (A) Alignment of human and zebrafish YPEL3 protein variants. The YPEL3 gene encodes two transcript variants: a long variant (NM_031477) that gives rise to a protein composed of 157 amino acids (NP_113665.3) and a short variant (NM_001145524) that encodes a protein of 119 amino acids (NP_001138996.10. One zebrafish ypel3 transcript variant (NM_212790.1) has been characterized to date. It encodes a protein of 119 amino acids (NP_997955.1). TV1: Long human YPEL3 protein variant. TV2: Short human YPEL3 protein variant. danio: Ypel3 zebrafish protein. Blue arrowhead indicates the location of Val92 in the long human isoform. (B) Section of 80 hpf zebrafish larva showing ypel3 expression at the level of the spinal cord and motor nerve (red arrows). (C) Section of the same specimen at a more rostral level of the spinal cord. Note the absence of ypel3 in the ventral medial region. Scale bar: 25 μm.