Figure 3

Figure 3

Expression analysis of zebrafish bid in normal embryos and embryos infected with E. ictaluri by using qPCR. (A) Expression of bid in normal embryos. Thirty to 50 embryos were collected for each time point. Expression of bid was normalized to the housekeeping gene gapdh.(B) Expression of bid in embryos infected with E. ictaluri. The zebrafish larvae at 4 dpf were infected with 2 × 10⁸ CFU/ml E. ictaluri and were collected at 12, 24, and 48 h postinfection (hpi). The fold changes of bid in embryos infected with E. ictaluri at each time point were analyzed using the 2^-ΔΔCT method relative to the corresponding control group. Asterisks indicated that the expression level of bid in embryos infected with E. ictaluri was significantly higher than that of the control. **p < 0.01.