ZFIN Image: Lu et al., 2021, Figure 4

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Figure Caption

Figure 4

Expression of kras^V¹² with LPS treatment enhanced the increase in cell apoptosis and cell proliferation in the intestinal epithelium. (A,C) Immunofluorescence staining (red) was carried out in intestinal paraffin sections of WT (N = 20), WT/LPS (N = 20), kras+ (N = 20), and kras+/LPS (N = 20) zebrafish. (B,D) Immunofluorescence staining of caspase-3 showing (1) apoptosis and (2) PCNA as a marker for cell proliferation as well as (3) quantification of the number and percentage of positive cells. Differences among the variables were assessed using Student’s t-tests. Statistical significance: * p < 0.05, *** p < 0.001. Scale bar: 50 μm.

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