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Figure 1
Zebrafish Aqp0b has adhesive properties. (A) Adherent cell percentages are shown for homotypic pairs of stable cell lines expressing positive control rat AQP0 (rAQP0), negative control rat AQP1 (rAQP1), zebrafish Aqp0a or Aqp0b, as well as cells transfected with an empty vector (L-(−)). Each lane represents n = 6–8. (B) Similar expression levels of rat AQP0 and zebrafish Aqp0 proteins shown by Western blotting using an anti-human C-terminus AQP0 antibody, and an anti-β-actin antibody as the loading control. Sample blot, top panel; quantification, bottom panel (n ≥ 3). (C) Heterotypic adhesion tested between zebrafish orthologs. Aqp0 plated first is indicated at the bottom. Statistical differences are shown as * having p < 0.05 or as ** having p < 0.01.