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Fig. 1.1 (A) No difference in health or swim behavior due to differences in diets: statistically indistinguishable fish length (p = 0.28), spontaneous swim velocity (p = 0.30), and swim velocity in presence of prey (p = 0.39). (B) Virtual prey capture. No difference between prey-experienced and prey-naïve fish in spontaneous tail flicks, eye saccades or eye convergences in absence of virtual prey stimulus (p = 0.48, p = 0.29, p = 0.76, respectively). (C, D) No effect on prey capture performance of time of day (C) or initial number of paramecia (D). (E, F) No effect of diet on swimming. Spontaneous swimming velocity (E) and percent of time spent resting (F) are similar in fish fed paramecia, flakes or pureed brine shrimp + dry food (husbandry diet) for 2 hours or 6 hr/day (one-way ANOVA test for (E) spontaneous swimming velocity 2 hr, Fstat = 2.95, p = 0.06; spontaneous swimming velocity 6 hr, Fstat = 0.19, p = 0.83; one-way ANOVA test for (F) % time resting 2 hr, Fstat = 2.95, p = 0.06; % time resting 6 hr, Fstat = 0.29, p = 0.75). (G) Fish previously fed with paramecia (N = 16) and fish previously fed with flakes (N = 17) on day 5 and 6 dpf are exposed to flakes on day 7 dpf for 10 min. Flakes-fed fish show a significantly higher amount of eye convergence during the recorded period than paramecia-fed fish, p < 0.0001. A permutation test was used for all pairwise comparisons if not specified otherwise (see Materials and methods, Behavioral data analysis and statistics).