ZFIN Image: Fraher et al., 2021, Fig. 8

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Figure Caption

Fig. 8 Combined sup-optimal doses of RA and ECS modulating chemicals influence osteoclast activity. Zebrafish were treated from 12 to 14 dpf with 60% combined doses of RA + RIMO, DEAB + OLE, or DEAB + WIN and osteoclast activity was assessed with TRAP stain at 14 dpf (A–D). RA + RIMO treated zebrafish had reduced numbers of labeled osteoclasts compared with controls (A,B). DEAB + OLE or DEAB + WIN had increased stained osteoclasts (C,D). n = 8 for each treatment group. (Fig. 8C and D). These results demonstrate that combined modulations of RA and ECS signaling have quantifiable effect on the mineralization of bone, the formation of osteoblasts and the number of active osteoclasts in vivo.

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Reprinted from Molecular and Cellular Endocrinology, 529, Fraher, D., Mann, R.J., Dubuisson, M.J., Ellis, M.K., Yu, T., Walder, K., Ward, A.C., Winkler, C., Gibert, Y., The endocannabinoid system and retinoic acid signaling combine to influence bone growth, 111267, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.