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Fig. 3 RA and ECS signaling alter col10a1 expression in zebrafish. Zebrafish were treated between 56 and 72 hpf for RA (B) and 48–72 hpf with RIMO (C), DEAB (D), WIN (E), and OLE (F). At 72 hpf, WISH was performed to observe any changes in col10a1 expression (B–F) as compared to the control (A) at the opercle marked with a black arrow, or marked by an arrowhead in close-ups. RA (B) and RIMO (C) treatments increased col10a1 expression as compared to control (A). DEAB (D), WIN (E) and OLE (F) treatments decreased col10a1 expression as compared to control (A). n = 10 for each treatment group.
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Reprinted from Molecular and Cellular Endocrinology, 529, Fraher, D., Mann, R.J., Dubuisson, M.J., Ellis, M.K., Yu, T., Walder, K., Ward, A.C., Winkler, C., Gibert, Y., The endocannabinoid system and retinoic acid signaling combine to influence bone growth, 111267, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.