IMAGE

Fig. 7

ID
ZDB-IMAGE-210804-20
Antibodies
Source
Figures for Fang et al., 2021
Image
Figure Caption

Fig. 7 Inhibition of PRC1 components is associated with disruption of neutrophils development.

A WISH for lyz (3 dpf) after UNC3866 treatment showing lyz+ cells decreased after 50 μM UNC3866 treatment. Quantification of lyz+ cells in asxl1+/+ and asxl1−/− tails (scale bar, 200 μm; black boxes show enlarged images; asxl1+/+, n = 16, asxl1−/−, n = 18, asxl1+/+ T, n = 20, asxl1−/− T, n = 15; one-way ANOVA followed by LSD Fisher’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). T treated, ns not significant. WISH of lyz (3 dpf) after 10 μM PRT4165 treatment showing lyz+ cells decreased after PRT4165 treatment. Quantification of lyz+ cells in asxl1+/+ and asxl1−/− tails (scale bar, 200 μm; black boxes show enlarged images; asxl1+/+, n = 12, asxl1−/−, n = 15, asxl1+/+ T, n = 10, asxl1−/− T, n = 14; one-way ANOVA followed by LSD Fisher’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). T treated, ns not significant. B WISH for lyz (3 dpf) after bmi1a mRNA injection showing reduced lyz expression was rescued by bmi1a mRNA injection. Quantification of lyz+ cells in asxl1+/+ and asxl1−/− tails (about 0.1 ng mRNA/embryo; scale bar, 200 μm; black boxes show enlarged images; control: asxl1+/+, n = 12, asxl1−/−, n = 18; bmi1a: asxl1+/+, n = 18, asxl1−/−, n = 10; one-way ANOVA followed by LSD Fisher’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD; T treated, ns not significant). C Western blot of H2AK119ub, H3K4me3 and H3K27me3 in 2 dpf asxl1+/+ and asxl1−/− embryos and quantification of Western blot data (input embryos: about ten embryos/well, performed with three replicates; two-tailed Student’s t test, ns not significant; error bars, mean ± SD, internal control, H3).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Leukemia