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Figure 2—figure supplement 1. (a) As Figure 2a, example ‘red’ (top) and ‘green’ (middle) fluorescence channels acquired simultaneously, and merged (bottom) and (b) extracted fluorescence profiles in the two channels as indicated. Note the absence of any obvious ‘bleed-through’, indicating good spectral separation of the two fluorescence detection bands. (c) Z-scored recordings before scaling and denoising (mean ± SD). (d) Superimposed means of the data shown in (c).

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