Fig. 2

Fig. 2

Behavioral (A) and electrophysiological (B, C) antiepileptic activity of valproate (VPA), (±) fenfluramine [(±)-FFA], topiramate (TPM), stiripentol (STP), cannabidiol (CBD), clobazam (CLB), levetiracetam (LEV), carbamazepine (CBZ), phenytoin (PHT), and lamotrigine (LTG) in the zebrafish scn1Lab^−/− mutant model. (A) Locomotor activity of larvae pre-exposed to antiepileptic drugs (AEDs) for 22 h. Data were assessed over the total tracking period of 10 min and expressed as cm/100 s. Results were pooled from 3–4 independent experiments, with 207 larvae for each of the VHC-treated groups, and 22–31 larvae for each AED-treated group. (B, C) Noninvasive local field potential (LFP) recordings from the optic tectum of larvae pre-exposed to antiepileptic drugs (AEDs) for 22 h. Epileptiform discharges are quantified by the cumulative duration (mean ± SEM) (B) and frequency (mean ± SEM) (C) of events per 10-min recording. With 72 larvae for the VHC-treated group, 10–15 larvae for each AED-treated group. Statistical analysis: one-way ANOVA with Dunnett’s multiple comparison test (locomotor assay), Kruskal–Wallis testing with Dunn’s multiple comparisons (LFP measurements). Significance levels: ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001, ^****p ≤ 0.0001. WT wide type, VHC vehicle