ZFIN Image: Shanaka et al., 2021, FIGURE 4

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FIGURE 4

The sGULO enzyme activity and AA level in Wt and Tg fish. (A) Detection of sGULO activity in Tg and Wt fish. Whole zebrafish were homogenized and used to detect the endogenous enzyme activity. This experiment was performed using an Oxi Select AA detection kit. The production rate of AA in Wt and Tg fish was measured after the addition of gulonolactone. (B) Detection of the endogenous AA level in Wt and Tg fish. Whole zebrafish were homogenized and used to detect endogenous AA levels using an Oxi Select AA detection kit. The error bars represent the standard deviation associated with experimental triplicates. The statistical significance between the Wt and Tg groups was analyzed using a t-test, and the significance is indicated by an asterisk *p < 0.05.

Acknowledgments

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