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Figure 2 Effect of detergent (1% NP-40) and sonication on enzymatic activities relative to the control. The scale samples lysed in the buffer in the presence (+) or absence (−) of 1%NP40 and sonication. (A) Cathepsin K activity, (B) TRAP activity, and (C) ALP activity. Interactions between groups were assessed with one-way ANOVA. The significant statistical analyses were analyzed with Tukey’s multiple comparison test. Data are shown as the mean ± SEM (**** p ≤ 0.0001; *** p ≤ 0.001; ns: not significant). Error bars indicate the standard error of the mean.
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