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Figure 4 The GFP reporter was expressed in the embryos from zebrafish transgenic line huORFZ exposed to various stresses. (A) The huORFZ embryos without any treatment displayed no GFP signal during 96 hpf served as negative control. (B,C) The heavy metal-containing chemicals, including AlCl3 and CuSO4, were used individually to treat huORFZ embryos at 72 hpf and observed at 96 hpf [118]. (B) For AlCl3-treated huORFZ embryos, GFP signals were observed in the brain and muscles. (C) For CuSO4-treated huORFZ embryos, GFP signal was observed in the skin cells. (D,E) The huORFZ embryos at 72 hpf were individually treated with heat shock or 1.5% alcohol and then observed the GFP signal during 96 hpf. Although GFP was induced to express by both stresses at the CNS, the expression patterns of them were distinct. (E) Alcohol-treated huORFZ embryos displayed weak GFP signal at the optic tectum (OT).