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Figure 1 Anaesthesia depends on time and bodyweight of zebrafish. (A) Schematic representation of experimental design. Recovery was tested after the indicated time in Tricaine plus 2 min expected surgery time. (B) Testing the effect of Tricaine concentrations (anaesthesia, rows) and durations (second column) on time to loss of tail reflex—stage 3 (third column) and after (fourth column) +2 min surgery simulation. The zebrafish mortality was noted for each group. (C) Time required to recover after 120 (white bar), 150-(grey bar) or 180 s (black bar) in 0.02, 0.03 or 0.04% Tricaine is depicted, respectively. (D) Time needed to reach stage 3 anaesthesia was measured for each zebrafish weight-class (300–499 mg, 500–699 mg or 700–899 mg). Statistical analysis used include (C) two-way ANOVA and (D) one-way ANOVA with Tukey’s multiple post-test (** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).