ZFIN Image: Koyama et al., 2021, Figure 1.

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Figure Caption

Figure 1.

Establishment of Tg fish that express botulinum toxin in GCs or PCs. Sagittal sections of adult gSA2AzGFF152B;Tg(UAS:BoTxBLC-GFP) (A–G), Tg(cbln12:Gal4FF);Tg(UAS:BoTxBLC-GFP) (H–N), and Tg(aldoca:BoTxBLC-GFP) (O–U) brains were stained with anti-GFP (green), and anti-Neurod1, or anti-parvalbumin 7 (Pvalb7, magenta) antibodies. A–C, H–J, O–Q, Cerebellum region. D–F, K–M, R–T, High-magnification views of the boxes in A, H, O. G, N, U, Low-magnification views. Cb, cerebellum; CCe, corpus cerebelli; Di, diencephalon; GL, granular layer; Hb, hindbrain; Hyp, hypothalamus; LCa, lobus caudalis cerebelli; ML, molecular layer; PCL; PC layer; SM, stratum marginale; Tel, telencephalon; TeO, tectum opticum; TL, torus longitudinalis; Vam, medial division of valvula cerebelli. Scale bars: 400 μm (A; applies to A–C, H–J, O–Q), 200 μm (D; applies to D–F, K–M, R–T), 1 mm (G, N, U).

Acknowledgments

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