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Figure 4.2 Distinct Erk activity between ablated and adjacent ISV ECs 3 hpa. (A–I’) Lateral spinning disc confocal images of ISV endothelial cells (ECs) in 4 days post-fertilisation (dpf) EC-EKC larvae at indicated timepoints. Images (A-I) show fli1aep:EKC expression, while images (A’-I’) show the nuclear fli1aep:EKC intensity. (J–M’) Erk-signalling is activated in ablated, but not adjacent ISV endothelial cells (ECs) at 3 hours post-ablation (hpa). Lateral spinning disc confocal images of ablated and adjacent ISV ECs in 4 dpf EC-EKC larvae before (J–K’) and 3 hr following vessel wounding (L–M’). Images (J-M) show fli1aep:EKC expression, while images (J’-M’) show the nuclear fli1aep:EKC intensity. Images (K) and (M) are higher magnification images of the yellow boxes in images (J) and (L). White circle in image (L) shows the wounded site. Arrows indicate the first (white), second (yellow), third (green), fourth (red), and fifth (orange) ECs from the wounded site. (N) Quantification of post-/pre-ablation nuclear EKC intensity of ECs in non-ablated control ISVs (27 ECs, n = 9 larvae), ablated ISVs (42 ECs, n = 14 larvae), and adjacent ISVs (42 ECs, n = 14 larvae) 3 hpa. ISV: intersegmental vessel. Statistical test: Kruskal-Wallis test was conducted for graph (N). Error bars represent standard deviation. Scale bars: 20 μm for image (A), 20 μm for images (J) and (K).