ZFIN Image: Okuda et al., 2021, Figure 3.2

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Figure Caption

Figure 3.2 Rapid Erk activation is largely restricted to wounded and adjacent ISV ECs.

(A–B’) Lateral confocal images of 4 days post-fertilisation (dpf) EC-EKC larvae pre-ablation (A,A’) and 15 min post-ablation (mpa) (B,B’). Immediately adjacent ISV endothelial cells (ECs) show rapid Erk activation, while Erk activity in second and third adjacent ISV ECs is largely unchanged. White dotted line shows the wounded site. (C) Quantification of post-/pre-ablation nuclear EKC intensity at 15 mpa in ECs of non-ablated control ISVs (30 ECs, n = 10 larvae), and adjacent (27 ECs, n = 9 larvae), second adjacent (27 ECs, n = 9 larvae), and third adjacent ISVs (27 ECs, n = 9 larvae). DA, dorsal aorta; ISV: intersegmental vessel. Statistical test: Kruskal-Wallis test was conducted for graph (C). Error bars represent standard deviation. Scale bar: 50 μm for image (A)

Acknowledgments

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