|
Figure 4 Effect of Euphorbiaceae compound B (ECB) on metastasis of melanoma cells in zebrafish xenografts. Zebrafish embryos were injected with A2058 cells at 48 hpf and observed at 6 hpi, 24 hpi, and 48 hpi, respectively. (a) Not injected. (b–d) A2058 cells were injected into zebrafish embryos at 48 hpf and observed at 6 hpi, 24 hpi, and 48 hpi, respectively. (e) A2058 cells were injected into zebrafish embryos and treated with 20 µg/mL ECB for 24 h and observed at 48 hpi. (f) A2058 cells were injected into zebrafish embryos and treated with 1 µM PLX4720 (PLX) for 24 h and observed at 48 hpi. (g) The area of CM-DiI labeling (melanoma cell) in the zebrafish embryos. Scale bar = 200 µm. indicates a significant difference (p<0.001).