ZFIN Image: Cheng et al., 2021, FIGURE 2

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Figure Caption

FIGURE 2

Label-free quantitative proteomics of HEK293 cells transfected with different cyclin F mutations (wild type, K97R, S195R, S509P, R574Q, S621G). (A) Hierarchical group clustering of proteome profiles from transfected cells showed ALS-specific and non-ALS mutations grouped together. Immunoblot analysis (B) and LFQ proteomics (C) of relatively equal cyclin F expression levels in transiently transfected HEK293 cells. (D) Two-dimensional principle component analysis (PCA) reflects the data quality and similarities of the proteome profiles between each biological replicate that are grouped by cyclin F mutations. Figures (A,C,D) were generated using Proteome Discoverer 2.3 (Thermo).

Acknowledgments

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