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Figure 2
(A) Align the WT and mutant sequence. (B) Blast the aligned WT sequence and determine the base numbers altered in the mutant. (C) Transfer these coordinates to the ZebraShare submission workbook. Descriptions become more complex for combined insertion/deletions alleles (InDels) and for alleles with multiple mutation sites due to use of multiple guide RNAs.
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Full text @ Peer J.