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Figure 3 Comparison of the larvae photomotor response (LPMR) for zebrafish larvae after treated with different doses of arecoline. LPMR of 120 hpf zebrafish after 1-day exposure of 0 ppm (black), 0.001 ppm (red), 0.01 ppm (blue), 0.1 ppm (green), and 1 ppm (purple) arecoline. Three LPMRs were measured. The data are expressed as means ± SD of total distance travelled of each following period. Data were analyzed by two-way ANOVA with the Geisser-Greenhouse correction. Each treated group was compared with the control group (n = 48 for the control, 0.01 and 1 ppm arecoline groups; n = 47 for 0.001 ppm arecoline group; n = 45 for 0.1 ppm arecoline group, ** p < 0.01).